G-circulating liposomes can alter their pharmacokinetic pattern and decrease MPS uptake (four). Furthermore, liposomes that have been modified with glycolipid-targeted ligandsare promising as a long-circulating and tumor-targeting carrier system (five). Asialoglycoprotein receptor (ASGPR) is often a high-capacity galactose-binding receptor expressed on the surface of hepatocytes (6) that possesses qualities that will particularly recognize glycoproteins that possess exposed terminal galactose or lactose residues (7). Liposomes modified with galactose derivatives might be recognized and bound by ASGPR in liver parenchyma cells, and significantly enhance the liver-targeted effect of liposome drugs (8). That is vital in the development of novel drugs in the remedy of hepatic carcinoma (9). Consequently, liposomes modified with galactose esters not simply stay away from the uptake of MPS, but also strengthen the targeting impact of liposomal carriers. In our previous study (10), 6-O-acyl-D-galactose esters have been synthesized by the enzymecatalyzed esterification of D-galactose and vinyl stearate (Fig. 1). The anti-cancer drug docetaxel was used as a model drug, and docetaxel liposomes modified with 6O-acyl-D-galactose esters (Gal-DOC-L) have been successfully prepared as a novel formulation for liver cancer therapy (11). Within the present study, the pharmacokinetic traits of docetaxel following intravenous administration of Gal-DOC-L in rabbits was investigated, plus the final results have been compared with docetaxel injection (DOC-I). Components and techniques Supplies. Docetaxel (purity, 99 ) was bought from the Beijing Norzer Pharmaceutical Co., Ltd. (Beijing, China). Norethisterone was bought in the National Institute for the Control of Pharmaceutical and Biological Merchandise (Beijing, China). DOC-I was bought in the Jiangsu Hengrui Medicine Co., Ltd. (Jiangsu, China). Gal-DOC-L have been ready in our laboratory (Division of Pharmaceutics, School of Chinese Materia Medica, Guangzhou University of Classic Chinese Medicine, Guangzhou, Guangdong, China).Sulforaphane uses Methanol and acetonitrile from the Shandong Yuwang Industrial Co.Methyl 4-bromo-2-naphthoate Purity , Ltd. (Shandong, China) had been of chromatographic grade.PMID:33682914 All other reagents had been of analytical grade and applied without further purification. Instruments. The Dionex high-performance liquid chromatography (HPLC) system consisted of a PDA-100 detector, a P680 pump and an ASI-100 autosampler (Dionex, Sunnyvale,Correspondence to: Professor Yi Cheng, College of ChineseMateria Medica, Guangzhou University of Traditional Chinese Medicine, 232 East of Waihuan Road, University Town, Guangzhou, Guangdong 510006, P.R. China E-mail: yicheng81@126Keywords: pharmacokinetics, docetaxel liposomes, 6-O-acyl-D-galactose, high-performance liquid chromatographyWU et al: PHARMACOKINETICS OF DOCETAXEL LIPOSOMESCA, USA). The chromeleon chromatography workstation software program was utilized for data acquisition and processing. Chromatographic conditions. Chromatographic separation was carried out on a Diamonsil C18 column (250×4.six mm, 5 ; Dikma Technologies, Inc., Radnor, PA, USA) using a EasyGuard Cl8 Security guard column (8×4.0 mm internal diameter; Dikma Technologies, Inc.) maintained at 30 . The mobile phase consisted of acetonitrile:water (55:45, v/v), at a flow price of 1.0 ml/min, plus the wavelength was set at 230 nm. Experimental animals. New Zealand rabbits were purchased in the Laboratory Animal Center of Guangzhou University of Chinese Medicine (Guangzhou, Guangdong, C.