Product Name :
SKP2 Rabbit Polyclonal Antibody
Predicted band size :
48 kDa
Observed band size :
Synonyms:
CDK2/Cyclin A associated protein p45 antibody Cyclin A/CDK2 associated protein p45 antibody Cyclin-A/CDK2-associated protein p45 antibody F box protein Skp2 antibody F box/LRR repeat protein 1 antibody F-box protein Skp2 antibody F-box/LRR-repeat protein 1 antibody FBL 1 antibody FBL1 antibody FBXL 1 antibody 展开
Function :
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
KLH conjugated synthetic peptide derived from human Skp2 351-424/424
Species reactivity:
Human, Mouse, Rat, Cow, Rabbit
Verify application:
WB, ELISA, IHC-P, IF, ICC
Coupling :
unconjugated
Clone number:
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at -20℃ for one year.Buy2-Bromonaphthalen-1-amine Avoid repeated freeze/thaw cycles.Buy1805526-89-9 The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃.PMID:33645443 When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer solution:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
48 KDa
Positive control :
293T Cell Lysate, Hela cell, human thyroid gland carcinoma tissue.