Ive harm in many oxidative stressrelated illnesses. In nephrotic disease, consumption of medicinal plant nutrients in conventional herbal medicine offers therapeutic advantages. This study was conducted to discover the possibility of prophylactic impact of AJDAE in improving doxorubicininduced nephrotoxicity in Wister albino rats. Additionally, molecular docking was utilised to determine and evaluate the interaction among the active compounds and also the observed biological activity by means of molecular docking.(10g) was drenched in 100ml of double distilled water for 18h and mixed up in a blender at a room temperature (225 ) (Abdelaziz Ali, 2014). Ajwa date aqueous extracts were sieved and centrifuged (Hettich ZENTRIFUGEN D7200 Tuttlingen Kind 1200 220V Benchtop Centrifuge) at 4 and 5000 g for 15min. The supernatants were withdrawn and stored at 4 till usage, and also the precipitant was removed. Fresh AJDAE was prepared each day all through the experiment days (Vayalil, 2002) just prior oral administration by utilizing intragastric gavage throughout the progress on the experimentation.2.2 | ChemicalsDoxorubicin vial was obtained from EBEWE Pharma Ges.m.b.H “Ebewe” (50mg/25ml Nfg. KG, A4866Unterach). Urea (URE120240), creatinine (CRE106240), calcium (CAL103120), phosphorous (PH123100), and uric acid (UA121240) had been obtained from EGY CHEM LAB Technology. Superoxide dismutase, glutathione peroxidase, glutathioneStransferase, glutathione reductase, catalase, and malonaldehyde competitive enzyme immunoassay method had been bought from Bioassay Technologies Laboratory, Shanghai Korain Biotech Co. DNA tissue extraction kit was supplied by Qiagen, DNeasy, RNeasy, QIAGEN Group. Top Vision AgaroseR0491, PBS131487 0, EDTAE1161, Tris AcetateEDTA buffer (TEAT8280), hematoxylin, and eosin had been supplied from Merck, SigmaAldrich.two.three | Chromatographic analysis of Ajwa date utilizing GCMSGas chromatography ass spectrometry (GCMS) chromatographic analysis of Ajwa date was performed using Agilent Technologies 7890B GC Systems combined with 5977A Mass Selective Detector.1415559-47-5 Chemical name Capillary column (HP5MS Capillary; 30.2-Methyl-2-azaspiro[3.3]Heptan-6-ol web 0 m.PMID:33420518 25mm ID.25m film) and helium as a carrier gas using a rate of flow of 1.7 ml/min with 1 l injection were also used. Evaluation of the sample was carried out withholding the column initially for four min at 40 postinjection, and after that the temperature was elevated to 300 (20 /min heating ramp) along with a 3.0min hold. The injection was completed in splitless mode at 300 . MS scan range was (m/z) 5050 atomic mass units under electron impact ionization (70eV).2 | M ATE R I A L S A N D M E TH O DS two.1 | Ajwa date (Phoenix dactylifera L.) aqueous extract preparationAjwa date was bought in the date industry in Jeddah, KSA; established and recognized by a professor of plant taxonomy; and banked inside the Herbarium of Biological Sciences Division, KAU (specimen voucher number: P. dactylifera L. #PD17569). The flesh of Ajwa date2.four | Silylation agent: BSA N, OBis (trimethylsilyl) acetamideThe reaction was carried out by adding 100l of BSA plus volume of the sample right after extraction and heating in water bath at 70 for 2h and after that injected into GCMS below the above conditions. The constituents were determined by mass fragmentations together with the NIST mass spectral search system for the NIST/EPA/NIH mass spectral library (June 2014).|BAOTHMAN et al.two.five | Experimental designThis study was approved by the Investigation Ethics Committee (REC), Faculty of Medicine, King Abdul Aziz University (KAU). Twomon.