Product Name :
WEE1 Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
DKFZp686I18166 antibody EC 2.7.10.2 antibody FLJ16446 antibody MGC105683 antibody OTTHUMP00000231338 antibody OTTHUMP00000231339 antibody Wee 1 antibody WEE 1 homolog 1 (S. pombe) antibody WEE1 antibody WEE1 homolog (S. pombe) antibody 展开
Function :
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ‘Tyr-15’. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ‘Tyr-15’ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
KLH conjugated synthetic peptide derived from human WEE1 151-250/646
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P, IF, ICC
Coupling :
unconjugated
Clone number:
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at -20℃ for one year.Buy872088-06-7 Avoid repeated freeze/thaw cycles.2,4-Dichloro-6-ethoxyquinazoline custom synthesis The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃.PMID:33529537 When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer solution:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
72 KDa
Positive control :