Product Name :
Phospho-Chk1 (S296) Recombinant Rabbit Monoclonal Antibody [SN06-50]
Predicted band size :
54 kDa
Observed band size :
54 kDa
Synonyms:
C85740 antibody Cell cycle checkpoint kinase antibody Checkpoint , S. pombe, homolog of, 1 antibody Checkpoint kinase 1 antibody Checkpoint kinase 1 homolog (S. pombe) antibody CHEK 1 antibody Chek1 antibody Chk 1 antibody Chk1 antibody CHK1 checkpoint homolog (S. pombe) antibody 展开
Function :
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser296 of human Chk1
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P, IF-Cell
Coupling :
unconjugated
Clone number:
SN06-50
RRID:
AB_3070056
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.3-Bromo-4-methylpyridin-2-ol supplier 4), 0.Price of 6-Bromoquinoline-3-carbaldehyde 05% BSA, 40% Glycerol.PMID:33666148 Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 54 kDa
Positive control :
HEK-293 treated with 200nM Calyculin A for 1 hour cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate, human tonsil tissue, human breast cancer tissue, HEK-293 cells treated with 100nM Calyculin A for 30 minutes.